Development of an efficient protocol for the production of deer embryos by in vitro fertilization: Application of sexed semen

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The general objective of this project is to develop a suitable protocol for in vitro fertilization in deer and adapt to the use of sexed sperm samples in this species. For this we propose to develop the following specific objectives:

1. To study the ideal time to capacitate spermatozoa and develop defined medium for the capacitation;

2. To determine the effect of oxygen tension during maturation and in vitro fertilization of oocytes on embryonic development;

3. To assess the effect of the absence or presence and the optimal time of addition of fetal bovine serum for in vitro culture of embryos on embryonic development;

4. To study conditions and optimal capacitation time for sexed sperm and the optimal concentration of sexed sperm in IVF.

Project number: AGL2013-48421-R. Financed by: Ministerio de Economía y Competitividad. Participants: UCLM.

Fondo europeo de desarrollo regional 

 

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